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Åke Lernmark

Principal investigator

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Analysis of islet cell antibodies on frozen sections of human pancreas

Author

  • B. Marner
  • Å Lernmark
  • J. Nerup
  • J. L. Molenaar
  • C. W. Tuk
  • G. J. Bruining

Summary, in English

The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p < 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of frozen sections, in the indirect immunofluorescence assay.

Publishing year

1983-08-01

Language

English

Pages

93-96

Publication/Series

Diabetologia

Volume

25

Issue

2

Document type

Journal article

Publisher

Springer

Keywords

  • assay precision
  • assay reproducibility
  • indirect immunofluorescence
  • insulin-dependent diabetes
  • Islet cell antibodies

Status

Published

ISBN/ISSN/Other

  • ISSN: 0012-186X