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Åke Lernmark

Principal investigator

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Block in insulin release from column-perifused pancreatic β-cells induced by islet cell surface antibodies and complement

Author

  • T. Kanatsuna
  • A. Lernmark
  • A. H. Rubenstein
  • D. F. Steiner

Summary, in English

Dispersed rat pancreatic islet cells were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused with an antiserum containing islet cell surface antibodies. The release of radioactive chromium from prelabeled cells, as a measure of cell membrane permeability, was not affected by cell surface antibodies alone, but increased dramatically in the presence of complement. While there was an eightfold increase in glucose-stimulated insulin release from β-cells exposed to control serum and complement, insulin release was completely blocked from β-cells exposed to islet-cell-specific antibodies and complement. These findings suggest that islet cell surface antibodies can mediate complement-dependent cytotoxicity.

Publishing year

1981-01-01

Language

English

Pages

231-234

Publication/Series

Diabetes

Volume

30

Issue

3

Document type

Journal article

Publisher

American Diabetes Association Inc.

Topic

  • Cell and Molecular Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0012-1797