Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Author

  • Giovanna Contreas
  • Ole D. Madsen
  • Henrik Vissing
  • Åke Lernmark

Summary, in English

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Publishing year

1986-12-04

Language

English

Pages

135-139

Publication/Series

Journal of Immunological Methods

Volume

95

Issue

1

Document type

Journal article

Publisher

Elsevier

Keywords

  • (BB rat)
  • Hybridoma screening
  • Islet cell surface antibody

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-1759