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Åke Lernmark

Principal investigator

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Islet β-cytotoxic monoclonal antibody against glycolipids in experimental diabetes induced by low dose streptozotocin and Freund's adjuvant

Author

  • M. Ziegler
  • S. Teneberg
  • S. Witt
  • B. Ziegler
  • B. Hehmke
  • K. D. Kohnert
  • J. Egeberg
  • K. A. Karlsson
  • A. Lernmark

Summary, in English

Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 ± 0.1 to 22.1 ± 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromatograms showed no binding of the K56aF3 antibody to glycolipids prepared from relevant cells. However, testing against a series of glycolipids of various non-pancreatic origins showed a preferential binding to a nine-sugar glycolipid isolated from human erythrocytes carrying an unusual blood group A determinant (type 3). It is suggested that this mAb may be associated with the development of diabetes following a combination of polyclonal activation and non-diabetogenic doses of streptozotocin.

Publishing year

1988-01-01

Language

English

Pages

4144-4150

Publication/Series

Journal of Immunology

Volume

140

Issue

12

Document type

Journal article

Publisher

American Association of Immunologists

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-1767