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Åke Lernmark

Principal investigator

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Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies

Author

  • E. Bonifacio
  • A. Lernmark
  • R. L. Dawkins
  • co-authors

Summary, in English

In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance.

Publishing year

1988-01-21

Language

English

Pages

83-88

Publication/Series

Journal of Immunological Methods

Volume

106

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Clinical Laboratory Medicine

Keywords

  • Insulin-dependent diabetes mellitus
  • Islet cell antibody
  • Standards

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-1759