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Åke Lernmark

Principal investigator

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Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells

Author

  • A. Lernmark
  • J. Sehlin
  • I. B. Täljedal
  • H. Kromann
  • J. Nerup

Summary, in English

Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.

Publishing year

1978-01-01

Language

English

Pages

25-31

Publication/Series

Diabetologia

Volume

14

Issue

1

Document type

Journal article

Publisher

Springer

Keywords

  • autoimmunity
  • Diabetes mellitus
  • insulin release
  • islet cell antibodies
  • pancreatic B-cells
  • rubidium accumulation
  • serum complement
  • serum cytotoxicity

Status

Published

ISBN/ISSN/Other

  • ISSN: 0012-186X