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Åke Lernmark

Principal investigator

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Antibodies directed against the pancreatic islet cell plasma membrane detection and specificity

Author

  • Å Lernmark
  • T. Kanatsuna
  • C. Patzelt
  • K. Diakoumis
  • R. Carroll
  • A. H. Rubenstein
  • D. F. Steiner

Summary, in English


Rabbits were immunised with suspensions of viable, insulin-producing islet cells prepared from collagenase-isolated rat or ob/ob mouse pancreatic islets. Antibodies reactive with the surface of dispersed rat islet cells were present in both the rabbit anti-rat and the rabbit anti -ob/ob mouse islet sera as revealed by indirect immunofluorescence or by a radioligandassay using
125
I-Protein A as a measure of cell bound IgG. In a competition assay the binding of
125
I-Protein A was displaced in a concentration dependent manner by non-radioactive Protein A. Maximal displacement was found at concentrations of Protein A higher than 0.1 μg. added to 10
5
islet cells. Although not always detected by immunofluorescence there was a several-fold increase above normal rabbit serum of
125
I-Protein A-binding to rat hepatocytes and spleen lymphocytes incubated with the islet cell antisera. Conversely, rabbit antisera against rat spleen lymphocytes or against a rat liver plasma membrane preparation reacted with rat islet cells. The rabbit anti-rat islet cell antiserum was absorbed to both spleen lymphocytes and hepatocytes until there was no binding of
125
I-Protein A to either cell type. Islet specific antibodies were still present since this doubly absorbed antiserum induced cell surface immunofluorescence as well as
125
I-Protein A-binding to rat islet cells. It is concluded that apart from common antigenic determinants immunisation with viable islet cells induces formation of antibodies directed against specific islet cell surface components.

Publishing year

1980-11-01

Language

English

Pages

445-451

Publication/Series

Diabetologia

Volume

19

Issue

5

Document type

Journal article

Publisher

Springer

Keywords

  • autoimmunity
  • cell surface antigens
  • cell surface immunofluorescence
  • cell suspensions
  • diabetes mellitus
  • islet cell surface antibodies
  • Pancreatic islet cells
  • Protein A radioassay

Status

Published

ISBN/ISSN/Other

  • ISSN: 0012-186X