Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release
Summary, in English
Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.
- ISSN: 0302-2137