Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

Direct streptozotocin toxicity on dispersed mouse islet cells determined by [ 51]CR-release

Author

  • H. Kromann
  • M. Christy
  • J. Egeberg
  • A. Lernmark
  • J. Nerup
  • H. Richter-Olesen

Summary, in English

Dispersed islet cells were prepared from collagenase-isolated lean mouse pancreatic islets by Dispase-II and subsequent mechanical treatment in calcium depleted media. An average yield of 600 cells per islet was obtained, 84% of the cells being β-cells. Cells were incubated with radioactive chromium as a marker of cell viability. Optimal labelling of 1-2 cpm per cell was obtained by incubating 10 5 cells with 10 6 cpm of [ 51]Cr for 90 min. When islet cells were incubated with streptozotocin, this drug induced [ 51]-Cr-release after a lag time of 2-4 hours. Furhermore, a positive correlation between streptozotocin concentrations and [ 51]Cr-release was found. This assay of cytotoxicity was highly reproducible and might be applicable in the study of other β-cell damaging agents or autoimmune phenomena in the pathogenesis of diabetes.

Publishing year

1980-12-01

Language

English

Pages

322-328

Publication/Series

Medical Biology

Volume

58

Issue

6

Document type

Journal article

Publisher

Medical Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0302-2137