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GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination

Author:
  • L. M. Bekris
  • R. A. Jensen
  • E. Lagerquist
  • T. R. Hall
  • Carl-David Agardh
  • Corrado Cilio
  • ÅsaLinda Lethagen
  • Åke Lernmark
  • J. A. Robertson
  • C. S. Hampe
Publishing year: 2007
Language: English
Pages: 521-526
Publication/Series: Diabetic Medicine
Volume: 24
Issue: 5
Document type: Journal article
Publisher: Wiley-Blackwell

Abstract english

Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern.

Keywords

  • Endocrinology and Diabetes
  • autoimmunity
  • therapy
  • Type 1 diabetes

Other

Published
  • Diabetes - Cellular Autoimmunity
  • Genomics, Diabetes and Endocrinology
  • Diabetes and Celiac Unit
  • ISSN: 1464-5491
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

+46 40 39 19 01

+46 70 616 47 79

60:11:015

Jan Waldenströms gata 35, Malmö

33

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00