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Åke Lernmark

Principal investigator

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Cytotoxic effects of a thiol reactive ferritin compound on isolated pancreatic β cells and the protective action of L cysteine

Author

  • G. D. Bloom
  • R. Gustafsson
  • A. Lernmark

Summary, in English

The effects of an electron dense thiol reagent, Hg phenylazo ferritin, on β cell ultrastructure was studied in cell suspensions from mouse pancreatic islets. This type of thiol reagent induced severe damage in the β cells. The ferritin particles were traced with the electron microscope and found to be bound to the plasma membrane as well as to various cytoplasmic structures. The β cell cytotoxic action of Hg phenylaxo ferritin was inhibited by L cysteine. The data conform with the assumption that there are highly reactive SH groups on the β cell surface, whose blocking may induce conformational changes in the plasma membrane and alter its permeability. The thiol reagent had no significant effect on insulin release from intact, microdissected mouse islets, probably owing to its poor penetrating ability.

Publishing year

1974-12-01

Language

English

Pages

244-249

Publication/Series

Medical Biology

Volume

52

Issue

4

Document type

Journal article

Publisher

Medical Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0302-2137