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Åke Lernmark

Principal investigator

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T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDMI susceptibility gene, LYP

Author

  • Daniel P. Gold
  • Samuel T. Shaikewitz
  • Deanna Mueller
  • Joan R. Redd
  • Karen S. Sellins
  • Anna Pettersson
  • Ake Lernmark
  • Donald Bellgrau

Summary, in English

Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4+ and CD8+ peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that previously described for mouse medullary thymocytes. In addition, cell surface staining of peripheral T cells from diabetes prone animals revealed a high frequency of Thyl+ cells, which is characteristic of both thymocytes and recent thymic emigrants. Following thymectomy, T cell responsiveness to a number of different stimuli is greatly reduced on a cell for cell basis as is the absolute number of surviving T cells. Taken collectively, our results suggest that the majority of the peripheral T cell pool in these diabetic prone rats consists of short lived, recent thymic emigrants which most likely also contain the effector cells required for initiation of diabetes.

Publishing year

1995-01-01

Language

English

Pages

149-161

Publication/Series

Autoimmunity

Volume

22

Issue

3

Document type

Journal article

Publisher

Taylor & Francis

Keywords

  • BB rats
  • Lymphopenia
  • T cell development

Status

Published

ISBN/ISSN/Other

  • ISSN: 0891-6934