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Åke Lernmark

Principal investigator

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A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2

Author

  • Peter D. Burbelo
  • Hiroki Hirai
  • Hannah Leahy
  • Ake Lernmark
  • Sten Ivarsson
  • Michael J. Iadarola
  • Abner Louis Notkins

Summary, in English

OBJECTIVE - Insulinoma-associated protein 2 (IA-2) is a major autoantigen in type I diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND METHODS - IA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation. RESULTS - Our study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R-2 = 0.805). CONCLUSIONS - The luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.

Department/s

  • Paediatric Endocrinology

Publishing year

2008

Language

English

Pages

1824-1826

Publication/Series

Diabetes Care

Volume

31

Issue

9

Document type

Journal article

Publisher

American Diabetes Association

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Paediatric Endocrinology

ISBN/ISSN/Other

  • ISSN: 1935-5548