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Åke Lernmark

Principal investigator

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Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane

Author

  • Bo Hellman
  • Lars Åke Idahl
  • Åke Lernmark
  • Janove Sehlin
  • Inge Bert Täljedal

Summary, in English

4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.

Publishing year

1973-12-01

Language

English

Pages

135-142

Publication/Series

The Journal of Membrane Biology

Volume

14

Issue

1

Document type

Journal article

Publisher

Springer

Status

Published

ISBN/ISSN/Other

  • ISSN: 0022-2631