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Åke Lernmark

Principal investigator

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A novel monoclonal antibody specific for the N-terminal end of GAD65

Author

  • Christiane S Hampe
  • Per Lundgren
  • Terri L Daniels
  • Lisa P Hammerle
  • Santica M. Marcovina
  • Åke Lernmark

Summary, in English

We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.

Publishing year

2001-02-01

Language

English

Pages

63-71

Publication/Series

Journal of Neuroimmunology

Volume

113

Issue

1

Document type

Journal article

Publisher

Elsevier

Keywords

  • Autoimmunity
  • GAD67
  • Immunoglobulin
  • Polymerase chain reaction
  • Type 1 diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0165-5728