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A novel monoclonal antibody specific for the N-terminal end of GAD65

Author:
  • Christiane S Hampe
  • Per Lundgren
  • Terri L Daniels
  • Lisa P Hammerle
  • Santica M. Marcovina
  • Åke Lernmark
Publishing year: 2001-02-01
Language: English
Pages: 63-71
Publication/Series: Journal of Neuroimmunology
Volume: 113
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.

Keywords

  • Autoimmunity
  • GAD67
  • Immunoglobulin
  • Polymerase chain reaction
  • Type 1 diabetes

Other

Published
  • ISSN: 0165-5728
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

+46 40 39 19 01

+46 70 616 47 79

60:11:015

Jan Waldenströms gata 35, Malmö

33

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00