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Åke Lernmark

Principal investigator

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Locus-specific detection of HLA-DQ and -DR antigens by antibodies against synthetic N-terminal octapeptides of the β chain

Author

  • Thomas Deufel
  • Anne Grove
  • Hans Kofod
  • Åke Lernmark

Summary, in English

Antibodies against synthetic peptides representing the class-II antigen HLA-DR and -DQ β chain N-terminal sequences were prepared in rabbits. The two octapeptides only share two amino acids and enzyme-linked immuno-assays showed the antisera only to bind to its own antigen. Both peptide antisera detected a 29 kDa component in immunoblots of Raji and AL-34 cell plasma membrane proteins separated by SDS gel electrophoresis. The binding of either N-terminal peptide antiserum was selectively inhibited only by the peptide used as antigen. Indirect immunofluorescence.analysis by flow cytofluorometry showed specific surface immunofluorescence in 1:100-1:1000 dilutions in lymphoblastoid and blood mononucleated cells. In the latter the binding was primarily confined to monocytes and a subpopulation of lymphocytes. It is concluded that locus-specific immunological reagents to distinguish between β chains of HLA-DR and -DQ have been prepared by the preparation by the production of antibodies against the N-terminal sequences of each polypeptide.

Publishing year

1985-09-23

Language

English

Pages

329-337

Publication/Series

FEBS Letters

Volume

189

Issue

2

Document type

Journal article

Publisher

Wiley-Blackwell

Keywords

  • ELISA
  • Flow cytofluorometry
  • HLA class II antigen
  • Immunoblotting bl]

Status

Published

ISBN/ISSN/Other

  • ISSN: 0014-5793