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Åke Lernmark

Principal investigator

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Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Author

  • D. Owerbach
  • A. Lernmark
  • L. Rask
  • P. A. Peterson
  • P. Platz
  • A. Svejgaard

Summary, in English

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

Publishing year

1983-01-01

Language

English

Pages

3758-3761

Publication/Series

Proceedings of the National Academy of Sciences of the United States of America

Volume

80

Issue

12

Document type

Journal article

Publisher

National Acad Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 0027-8424