Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here:

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

Susceptibility to insulin-dependent diabetes defined by restriction enzyme polymorphism of HLA-D region genomic DNA


  • D. Owerbach
  • B. Hagglof
  • A. Lernmark
  • G. Holmgren

Summary, in English

DNA fragments complementary to cloned sequences encoding HLA-D region class II antigen α- and β-chains were determined by clotting with DNA from HLA-typed members of 22 complete families, 12 of which had a proband with insulin-dependent diabetes mellitus (IDDM). Analysis of genotypes showed that the DNA sequences were linked to HLA-DR and permitted confirmation of recombinations in two families. Digestion with the restriction enzymes BamHI, EcoRI, and Pstl and hybridization with an HLA-D region β-chain cDNA probe confirmed a BamHI 3.7 kilobase (kb) fragment present at low frequency among diabetic individuals and a BamHI 3.2 kb fragment that was also decreased among the diabetic subjects compared with siblings (P < 0.05) as well as nonrelated control siblings (P < 0.02) and their parents (P < 0.01). BamHI 12.0 kb (P < 0.05) and 5.8 kb (P < 0.02, P < 0.02), EcoRI 20 kb (P < 0.05, P < 0.02), and Psti 6.0 kb (P < 0.05) fragments were more frequent in diabetic individuals compared with nonrelated control siblings or their parents, respectively. Analysis of individual haplotypes revealed that HLA-DR4-containing chromosomes were heterogeneous among controls but that the diabetic individuals showed a similar pattern of restriction fragment length polymorphism. Genomic blotting of blood lymphocyte DNA with a cDNA clone encoding the chain of HLA-D region class II antigens permits detection of fragments that are strongly associated with IDDM.

Publishing year












Document type

Journal article


American Diabetes Association Inc.




  • ISSN: 0012-1797