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Åke Lernmark

Principal investigator

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Inhibition of insulin release after passive transfer of immunoglobulin from insulin-dependent diabetic children to mice

Author

  • Annette Svenningsen
  • Thomas Dyrberg
  • Ivan Gerling
  • Åke Lernmark
  • Peter Mackay
  • Alexander Rabinovitch

Summary, in English

We used the mouse passive transfer model to test whether islet cell antibodies affect β-cell function. The immunoglobulin (Ig) fraction of plasma from 5 islet cell surface antibody-positive, newly diagnosed insulin-dependent diabetic children or of a pool of plasma from 12 normal subjects was injected daily (7–16 mg IgG/day) for 14 days into normal immunosuppressed BALB/c mice. Insulin secretory responses in the Ig-injected mice were then examined by perfusing the rodent pancreata in vitro. Insulin release induced by 20 mmol/liter Dglucose during 30 min of stimulation decreased from 900 ng insulin (median; range, 814–1138) from pancreata of mice injected with control Ig to 511 ng (range, 130–786) from pancreata of mice injected with diabetic Ig (P < 0.003). Both the initial peak and the sustained second phase of glucose-stimulated insulin release were depressed in 4 of the 5 pancreata from mice injected with diabetic Ig. These results indicate that circulating antibodies in diabetic children may alter β-cell function and possibly contribute to the pathogenesis of insulin-dependent diabetes.

Publishing year

1983-01-01

Language

English

Pages

1301-1304

Publication/Series

Journal of Clinical Endocrinology and Metabolism

Volume

57

Issue

6

Document type

Journal article

Publisher

Oxford University Press

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-972X