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Åke Lernmark

Principal investigator

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Rel B is an early marker of autoimmune islet inflammation in the BioBreeding (BB) rat

Author

  • Sabine Bieg
  • William T Simonson
  • Kristian Ellefsen
  • Åke Lernmark

Summary, in English

Because the development of insulitis and diabetes is predictable in Lyp/Lyp congenic BB rats, we have characterized early islet inflammation in these rats to determine the cell subsets involved in the onset of autoimmune insulitis. Pancreas sections from prediabetic Lyp/Lyp, Lyp/+ and +/+ rats were analyzed by immunohistochemistry. We found W3/25+ cells in the exo- and endocrine tissue from all three genotypes, but intraislet insulitis was never found in Lyp/+ or +/+ rats. The onset of massive, intraislet B- and T-cell infiltration in Lyp/Lyp rats was preceded by Rel B+ cells in and around the islets, followed by ED1+ monocytes/macrophages. Rel B+ cells were more frequent in the parafollicular cortex of pancreatic lymph nodes from Lyp/Lyp than from Lyp/+ and +/+ rats. In the Lyp/Lyp thymus, we found significantly increased expression of IL-12p40 messenger RNA (mRNA; p < 0.001), located in the Rel B-protein-rich corticomedullary junction. The NF-κB/Rel B complex specifically transactivates genes involved in antigen presentation in dendritic cells. Rel B+ cells in the islets may therefore mark the onset of autoimmune insulitis and antigen-specific activation of autoreactive T cells in the lymph nodes of diabetes prone Lyp/Lyp BB rats. In the thymus, Rel B+ cells may support the Lyp-dependent development of self-reactive thymocytes by activation of cytokine expression.

Publishing year

2000-01

Language

English

Pages

47-54

Publication/Series

Pancreas

Volume

20

Issue

1

Document type

Journal article

Publisher

Lippincott Williams & Wilkins

Keywords

  • Antigen-presenting cell
  • Congenic
  • Dendritic cell
  • Diabetes
  • Pancreas
  • T cell

Status

Published

ISBN/ISSN/Other

  • ISSN: 0885-3177