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Åke Lernmark

Principal investigator

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The dynamics of insulin release from mouse pancreatic islet cells in suspension

Author

  • Lars åke Idahl
  • Åke Lernmark
  • Janove Sehlin
  • Inge Bert Täljedal

Summary, in English

The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 μM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.

Publishing year

1976-01-01

Language

English

Pages

185-188

Publication/Series

Pflügers Archiv European Journal of Physiology

Volume

366

Issue

2-3

Document type

Journal article

Publisher

Springer

Keywords

  • β-Cells in suspension
  • Glucose oxidation
  • Insulin release
  • Intact pancreatic islets
  • Pancreatic β-cells

Status

Published

ISBN/ISSN/Other

  • ISSN: 0031-6768