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Åke Lernmark

Principal investigator

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Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

Author

  • Mieczyslaw Michalkiewicz
  • Teresa Michalkiewicz
  • Ruth A. Ettinger
  • Elizabeth A. Rutledge
  • Jessica M. Fuller
  • Daniel H. Moralejo
  • Brian Van Yserloo
  • Armand J. MacMurray
  • Anne E. Kwitek
  • Howard J. Jacob
  • Eric S. Lander
  • Åke Lernmark

Summary, in English

A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.

Publishing year

2004

Language

English

Pages

228-232

Publication/Series

Physiological Genomics

Volume

19

Issue

2

Document type

Journal article

Publisher

American Physiological Society

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 1094-8341