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Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

Author:
  • Mieczyslaw Michalkiewicz
  • Teresa Michalkiewicz
  • Ruth A. Ettinger
  • Elizabeth A. Rutledge
  • Jessica M. Fuller
  • Daniel H. Moralejo
  • Brian Van Yserloo
  • Armand J. MacMurray
  • Anne E. Kwitek
  • Howard J. Jacob
  • Eric S. Lander
  • Åke Lernmark
Publishing year: 2004
Language: English
Pages: 228-232
Publication/Series: Physiological Genomics
Volume: 19
Issue: 2
Document type: Journal article
Publisher: American Physiological Society

Abstract english

A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • ISSN: 1094-8341
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

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+46 70 616 47 79

60:11:015

Jan Waldenströms gata 35, Malmö

33

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00