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Åke Lernmark

Principal investigator

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Unique epitopes of glutamic acid decarboxylase autoantibodies in slowly progressive type 1 diabetes.

Author

  • Tetsuro Kobayashi
  • Shoichiro Tanaka
  • Minoru Okubo
  • Koji Nakanishi
  • Toshio Murase
  • Åke Lernmark

Summary, in English

Disease-specific epitope profiles of glutamic acid decarboxylase (GAD)65 autoantibodies (GAD65Ab) were studied in slowly progressive type 1 (insulin-dependent) diabetes mellitus (SPIDDM) and acute onset type 1 (insulin-dependent) diabetes mellitus (AIDDM) using seven kinds of GAD65/67 chimeric molecules. Sera obtained from Japanese SPIDDM (n = 17) and AIDDM (n = 46) patients followed prospectively were analyzed by immunoprecipitation, ELISA, and Western blotting. GAD65Ab in all SPIDDM samples reacted specifically with an N-terminal linear epitope located on the membrane anchoring domain between amino acids 17-51 and C-terminal conformational epitope between amino acids 443-585 of GAD65. The binding of GAD65Ab with N-terminal 83 residues in SPIDDM inversely correlated with the period in which insulin was not required. GAD65Ab in AIDDM did not react with N-terminal epitope located between amino acids 1-83, irrespective of the titer of GAD65Ab. A novel epitope of GAD65Ab in AIDDM residing

Publishing year

2003

Language

English

Pages

4768-4775

Publication/Series

Journal of Clinical Endocrinology and Metabolism

Volume

88

Issue

10

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 1945-7197