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Åke Lernmark

Principal investigator

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Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.

Author

  • Andrea D Carroll
  • Louis Scampavia
  • Dong Luo
  • Åke Lernmark
  • Jaromir Ruzicka

Summary, in English

This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection.

Publishing year

2003

Language

English

Pages

1157-1162

Publication/Series

Analyst

Volume

128

Issue

Sep

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 1364-5528