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Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus.

Author:
  • Andrea D Carroll
  • Louis Scampavia
  • Dong Luo
  • Åke Lernmark
  • Jaromir Ruzicka
Publishing year: 2003
Language: English
Pages: 1157-1162
Publication/Series: Analyst
Volume: 128
Issue: Sep
Document type: Journal article
Publisher: Royal Society of Chemistry

Abstract english

This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • ISSN: 1364-5528
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

+46 40 39 19 01

+46 70 616 47 79

60:11:015

Jan Waldenströms gata 35, Malmö

33

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00