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Åke Lernmark

Principal investigator

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Stimulation of insulin release by thiols

Author

  • Bo Hellman
  • Lars Åke Idahl
  • Åke Lernmark
  • Janove Sehlin
  • Inge Bert Täljedal

Summary, in English

The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mM D-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the β-cells.

Publishing year

1975-05-05

Language

English

Pages

101-109

Publication/Series

BBA - General Subjects

Volume

392

Issue

1

Document type

Journal article

Publisher

Elsevier

Status

Published

ISBN/ISSN/Other

  • ISSN: 0304-4165