Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

A β-cell glycoprotein of Mr 40 000 is the major rat islet cell immunogen following xenogenic immunisation

Author

  • S. Bækkeskov
  • Å Lernmark

Summary, in English

An antiserum (R2) was raised in a rabbit against dispersed Sprague Dawley rat islet cells. The R2 antiserum contains islet cell surface antibodies, which mediate complement-dependent cytotoxicity against islet cells resulting in a block of glucose induced insulin release. Immunoprecipitation and gel electrophoretic analysis showed that R2 specifically recognizes an Mr 40 000 glycoprotein present in both rat islet and rat insulinoma cells. This glycoprotein is amphiphilic in character and probably represents a pancreatic β cell specific plasma membrane component. The results support previous observations in mouse β cells that a plasma membrane glycoprotein of Mr 40K constitutes a major islet cell immunogen.

Publishing year

1984-07-01

Language

English

Pages

70-73

Publication/Series

Diabetologia

Volume

27

Issue

1 Supplement

Document type

Journal article

Publisher

Springer

Keywords

  • antiserum R2
  • Glycoprotein M 40 000
  • islet cell immunogen
  • rat
  • xenogenic immunisation

Status

Published

ISBN/ISSN/Other

  • ISSN: 0012-186X