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Åke Lernmark

Principal investigator

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Cation-activated phosphatase activities in islet cell plasma membrane preparations.

Author

  • A. Lernmark
  • D. A. Nielsen
  • A. U. Parman
  • J. Sehlin
  • D. F. Steiner
  • I. B. Täljedal

Summary, in English

Pancreatic islets from rats or ob/ob mice were homogenized and fractionated either by a two-step or one-step sucrose gradient centrifugation. A plasma membrane enriched fraction was obtained at a sucrose density of about 1.10. The distribution of the plasma membrane probe 125I-wheat germ agglutinin was parallel to that of other plasma membrane markers. Hydrolysis of Mg-ATP-gamma-3Pp in rat islet membranes was of high specific activity, but was little affected by K+ and/or Na+. K+-activated, ouabain-sensitive phosphatase activities were, on the other hand, readily demonstrated in both rat and ob/ob mouse islet membranes. The K+-activated hydrolysis of organophosphate indicators were markedly inhibited by ATP. The binding of 45Ca2+ to mouse islet plasma membranes was increased by ATP. Cation transport and the interaction of Ca2+ with the islet cell plasma membrane may be dependent on phosphoryl-transfer reactions with ATP as the physiological substrate.

Publishing year

1980-12-01

Language

English

Pages

55-61

Publication/Series

Hormone and Metabolic Research, Supplement

Volume

Suppl 10

Document type

Journal article

Topic

  • Cell and Molecular Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0170-5903