Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Åke Lernmark

Principal investigator

Default user image.

Scanning electron microscopy of surface changes on dispersed pancreatic β-cells following stimulation of insulin release

Author

  • A. Lernmark
  • B. Winblad

Summary, in English

The surface structure of isolated viable β-cells was studied by scanning electron microscopy. Suspensions of islet cells were prepared from the β-cell-rich islets of the ob/ob mouse. Cells incubated with and without D-glucose were fixed while in suspensions, filtered onto Nucleopore filters and prepared for scanning electron microscopy by the critical point drying procedure. After incubation in glucose free medium the cells were spherical with surface blebs and occasional small pits. Cells stimulated with 20 mM D-glucose and 0.1 mM 3-isobutyl-1-methyl-xanthine exhibited a two fold increase in the number of blebs and in insulin release. The increased number of blebs was still seen in a Ca++ deficient medium, and may, therefore, not necessarily reflect an insulin extrusion phenomenon. It is concluded that D-glucose induces alternations of the surface morphology in isolated β-cells in suspension.

Publishing year

1977-12-01

Language

English

Pages

141-147

Publication/Series

Medical Biology

Volume

55

Issue

3

Document type

Journal article

Publisher

Medical Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0302-2137