Pancreatic islet uptake of sucrose and urea in the absence and presence of glucose
Summary, in English
Microdissected pancreatic islets of ob/ob mice were used to study whether glucose induced insulin release is associated with changes of the apparent extracellular space. Glucose at a concentration of 20 mM had no measurable effect on the islet uptake of sucrose and urea. In the presence of these space markers glucose stimulated insulin release significantly. The results do not invalidate the hypothesis that stimulation of insulin release is associated with increased endocytosis in the β cells. They suggest, however, that endocytosis does not represent a significant source of error when measuring the islet uptake of compounds in relation to extracellular space markers.