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Åke Lernmark

Principal investigator

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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

Author

  • Hiroki Hirai
  • Junnosuke Miura
  • Yafang Hu
  • Helena Larsson
  • Karin Larsson
  • Åke Lernmark
  • Sten Ivarsson
  • Tianxia Wu
  • Albert Kingman
  • Athanasios G Tzioufas
  • Abner L Notkins

Summary, in English

The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.

Department/s

  • Paediatric Endocrinology
  • Diabetes and Celiac Unit

Publishing year

2008

Language

English

Pages

366-374

Publication/Series

Clinical Immunology

Volume

127

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Keywords

  • type 1 diabetes
  • secretory vesicles
  • proteome
  • phosphatase
  • protein tyrosine
  • IA-2
  • GAD65
  • autoantibodies
  • autoantigens

Status

Published

Research group

  • Paediatric Endocrinology
  • Diabetes and Celiac Unit

ISBN/ISSN/Other

  • ISSN: 1521-6616