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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

  • Hiroki Hirai
  • Junnosuke Miura
  • Yafang Hu
  • Helena Larsson
  • Karin Larsson
  • Åke Lernmark
  • Sten Ivarsson
  • Tianxia Wu
  • Albert Kingman
  • Athanasios G Tzioufas
  • Abner L Notkins
Publishing year: 2008
Language: English
Pages: 366-374
Publication/Series: Clinical Immunology1999-01-01+01:00
Volume: 127
Issue: 3
Document type: Journal article
Publisher: Elsevier

Abstract english

The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.


  • Immunology in the medical area
  • type 1 diabetes
  • secretory vesicles
  • proteome
  • phosphatase
  • protein tyrosine
  • IA-2
  • GAD65
  • autoantibodies
  • autoantigens


  • Paediatric Endocrinology
  • Diabetes and Celiac Unit
  • ISSN: 1521-6616
E-mail: ake [dot] lernmark [at] med [dot] lu [dot] se

Principal investigator

Diabetes and Celiac Unit

+46 40 39 19 01

+46 70 616 47 79


Jan Waldenströms gata 35, Malmö


Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00