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Åke Lernmark

Principal investigator

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Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

Author

  • Wei Hao
  • Linsong Li
  • Vivek Mehta
  • Åke Lernmark
  • Jerry P. Palmer

Summary, in English

Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.

Publishing year

1994-01-01

Language

English

Pages

558-562

Publication/Series

Pancreas

Volume

9

Issue

5

Document type

Journal article

Publisher

Lippincott Williams & Wilkins

Keywords

  • Glutamic acid decarboxylase
  • Insulin-dependent diabetes mellitus
  • β cell function

Status

Published

ISBN/ISSN/Other

  • ISSN: 0885-3177