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Åke Lernmark

Principal investigator

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Radioimmunoassay detects the frequent occurrence of autoantibodies to the mr 65,000 isoform of glutamic acid decarboxylase in japanese insulin-dependent diabetes

Author

  • A. Falorni
  • C. E. Grubin
  • I. Takei
  • A. Shimada
  • A. Kasuga
  • T. Maruyama
  • Y. Ozawa
  • T. Kasatani
  • T. Saruta
  • L. Li
  • A. Lernmark

Summary, in English

Glutamic acid decarboxylase antibodies (GAD65Ab) are common in new onset Caucasian insulin-dependent diabetic (IDDM) patients but it is unclear if this marker is also prevalent in patients of other ethnic backgrounds. We determined antibodies against human recombinant GAD in Japanese diabetic patients using a radioimmunoassay with competition between in vitro translated 35S-GAD65 and non-labelled recombinant human GAD65 (rhGAD65). GAD67 antibodies (GAD67Ab) were similarly analyzed but without antigen competition. In 73 Japanese diabetic patients, GAD65Ab were found in 11/16 (69% of patients with short-duration (less than 5 yrs) IDDM, 6/23 (26% with long-duration (5 or more yrs) IDDM and 10/20 (50% with slowly progressive diabetes. High GAD65Ab levels were associated with concomitant autoimmune diseases (p = 0.021). GAD67Ab were found in 4/16 (25% of patients with short-duration IDDM, 3/23 (13% with long-duration IDDM and 2/20 (10% with slowly progressive diabetes. In 14 non-insulin dependent diabetic (NIDDM) patients, GAD65Ab and GAD67Ab were not found (0/14) and 1/50 (2% healthy controls were positive in either assay. Among the GAD67Ab-positive samples, 8/9 (88% were also high level GAD65Ab positive, 7/9 (77% were displaced by an excess of rhGAD65 and the antibody levels correlated (r2 = 0.573; p = 0.003). Our data are consistent with a strong association of GAD65Ab also in Japanese IDDM, and suggest that, when present, GAD67Ab are frequently directed to epitope(s) common to GAD65 and GAD67.

Publishing year

1994-01-01

Language

English

Pages

113-125

Publication/Series

Autoimmunity

Volume

19

Issue

2

Document type

Journal article

Publisher

Taylor & Francis

Keywords

  • Autoantigen
  • Autoimmunity
  • Glutamic acid decarboxylase
  • Insulin-dependent diabetes
  • Radioimmunoassay

Status

Published

ISBN/ISSN/Other

  • ISSN: 0891-6934