Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.
- Centre for Analysis and Synthesis
Publishing year: 2003
Document type: Journal article
We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement.
- Analytical Chemistry
- Ultrasonic trapping
- Capillary electrophoresis
- Particle separation
- Concentration limit of detection
- ISSN: 0041-624X