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Perilipin and adipophilin expression in lipid loaded macrophages

Author:
  • Jenny Persson
  • Eva Degerman
  • Jan Nilsson
  • Marie Lindholm
Publishing year: 2007
Language: English
Pages: 1020-1026
Publication/Series: Biochemical and Biophysical Research Communications
Volume: 363
Issue: 4
Document type: Journal article
Publisher: Elsevier

Abstract english

Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved.

Keywords

  • Biological Sciences
  • PAT proteins
  • lipid droplet
  • foam cell
  • macrophage

Other

Published
  • Experimental Cardiovascular Research Unit
  • Insulin Signal Transduction
  • ISSN: 1090-2104
Eva Degerman
E-mail: eva.degerman [at] med.lu.se

Professor

Insulin Signal Transduction

+46 46 222 85 83

+46 70 885 83 62

BMC C1121b

66

Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00