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Insulin-induced translocation of protein kinase B to the plasma membrane in rat adipocytes

Author:
  • Olga Göransson
  • Jonny Wijkander
  • V Manganiello
  • Eva Degerman
Publishing year: 1998
Language: English
Pages: 249-254
Publication/Series: Biochemical and Biophysical Research Communications
Volume: 246
Issue: 1
Document type: Journal article
Publisher: Elsevier

Abstract english

Protein kinase B (PKB) has previously been shown to be activated in response to insulin and growth factor stimulation. The activation mechanism has been suggested to involve translocation of PKB to membranes, where it is phosphorylated and activated. Insulin-induced translocation of PKB has not been demonstrated in a physiological target cell. Therefore we have used the primary rat adipocyte to investigate insulin-induced translocation of PKB. In the presence of 1 nM insulin translocation of PKB was detected within 30 seconds and was blocked by wortmannin, a selective phosphatidylinositol 3-kinase inhibitor. This translocation was potentiated by the tyrosine phosphatase inhibitor vanadate. Subcellular localization studies revealed that PKB translocated to the plasma membrane.

Keywords

  • Biological Sciences
  • insulin
  • adipocyte
  • protein kinase B
  • plasma membrane
  • phosphatidylinositol 3-kinase

Other

Published
  • Insulin Signal Transduction
  • ISSN: 1090-2104
Eva Degerman
E-mail: eva.degerman [at] med.lu.se

Professor

Insulin Signal Transduction

+46 46 222 85 83

+46 70 885 83 62

BMC C1121b

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Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00