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Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements

Author:
  • T Kanno
  • Xiaosong Ma
  • Sebastian Barg
  • Lena Eliasson
  • Juris Galvanovskis
  • Sven Göpel
  • Max Larsson
  • Erik Renström
  • Patrik Rorsman
Publishing year: 2004
Language: English
Pages: 302-311
Publication/Series: Methods
Volume: 33
Issue: 4
Document type: Journal article
Publisher: Elsevier

Abstract english

This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.

Keywords

  • Endocrinology and Diabetes

Other

Published
  • Islet cell physiology
  • Islet cell exocytosis
  • Neurophysiology
  • Islet patophysiology
  • ISSN: 1095-9130
Erik Renström
E-mail: erik.renstrom [at] med.lu.se

Deputy head of department

Department of Clinical Sciences, Malmö

+46 40 39 11 57

+46 40 39 11 57

Principal investigator

Islet patophysiology

+46 40 39 11 57

+46 40 39 11 57

20-3-308

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Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00